Blues Brothers 2000 picks up 18 years after the brothers’ original “mission from God” left off, with Elwood Blues (Dan Aykroyd) getting out of prison and discovering that much has changed in the time he’s been away. His partner, Jake, is dead, his band is long gone and the orphanage where he grew up has been demolished. Even Curtis, the orphanage custodian who was the surrogate father to Jake and Elwood, has passed on, making Elwood’s only living relation Curtis’ bastard son Cabel (Joe Morton) – his almost-stepbrother. Cabel, a straight- as-an-arrow , by-the-book cop , doesn’t exactly acknowledge any family ties; he’s convinced that Elwood’s still up to no good.

Seeking guidance from his former teacher, Sister Mary Stigmata, Elwood finds her working as a hospital administrator with a problem of her own; she’s at the end of her rope with Buster (J. Evan Bonifant), a 10-year-old orphan seriously in need of a “mentor.”

Acknowledging that “the Lord works in mysterious ways,” Elwood soon realizes that he’s embarked on a whole new mission – to reassemble the old band, compete at Queen Mousette’s Battle of the Bands in Louisiana and set the wayward Buster on the path to redemption. With Elwood, that path leads them right to a strip joint where they hook up with bartender Mighty Mack McTeer (John Goodman), who knows a thing or two about the blues, himself. The three then hit the road in order to reunite the band, trying their best to stay ahead of Cabel and the cops, as well as the Chicago Chapter of the Moscow Mafia and a rabidly right-wing militia group.